Pregnancy Specific Protein B (PSPB) ELISA Kit is a commercially available enzyme-linked immunosorbent assay (ELISA) kit used to quantitatively measure the concentration of PSPB in human serum or plasma samples.
The kit typically includes pre-coated microtiter plate(s) with monoclonal antibodies specific to PSPB, along with a standard curve and a set of reagents necessary for the assay. The assay is based on the principle of competitive binding, where a known amount of PSPB present in the sample competes with a fixed amount of PSPB conjugated to an enzyme for binding to the PSPB-specific antibodies on the microtiter plate.
After a series of washing steps, a substrate solution is added, which reacts with the enzyme conjugate to produce a colorimetric signal that is proportional to the amount of PSPB in the sample. The absorbance of the signal is measured using a microplate reader, and the PSPB concentration in the sample is determined by comparing the signal to the standard curve.
The Pregnancy Specific Protein B ELISA Kit is commonly used in clinical research and diagnostic laboratories to detect PSPB, which is a marker of placental development and fetal well-being during pregnancy. The kit provides a fast, accurate, and reliable method for measuring PSPB levels in biological samples.
Bovine PSPB(Pregnancy Specific Protein B) ELISA Kit
Catalogue No. EB0004
Detection Method : Competitive ELISA, Coated with Antigen
Application : PSPB ELISA Kit allows for the in vitro quantitative determination of PSPB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size : 96T
Range : 0.781-50ng/ml
Sensitivity : < 0.469ng/ml
Species : Bovine
UniProt ID : Q29432
Storage : 2-8 ℃ for 6 months
Recovery : Matrices listed below were spiked with certain level of PSPB and the recovery rates were calculated by comparing the measured value to the expected amount of PSPB in samples.
Linearity : The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PSPB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CV(%) : Intra-Assay: CV<8%, Inter-Assay: CV<10%
This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase
supporter for sites on the Biotinylated Detection Antibody specific to target.
Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB
substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm.
The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.
Note : For Research Use Only